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缇庡浗椹惃璇稿澶у鍗氬＋姣曚笟锛岀航绾﹀ぇ瀛﹀尰瀛﹂櫌鍗氬＋鍚庯紝鐧惧ゥ璧涘浘姹熻嫃鍩哄洜鐢熺墿鎶鏈湁闄愬叕鍙歌懀浜嬮暱鍏兼荤粡鐞嗐傜幇浠讳腑鍏虫潙“娴峰垱鏉”鍒涙柊鍒涗笟澶ц禌璇勫鍜屽垱涓氬甯堛佸寳浜鍗忓拰鍖诲闄腑鍥藉尰瀛︾瀛﹂櫌璇勯槄浜恒傜幇浠诲紑鍙戝尯绉戝崗绗簩灞婂鍛樹細鍓富甯佷笂娴峰競鐢熺墿宸ョ▼瀛︿細绗竷灞婄悊浜嬩細鐞嗕簨鍜屽寳浜競瀹為獙鍔ㄧ墿瀛︿細绗崄灞婄悊浜嬩細鐞嗕簨銆
鐮旂┒鏂瑰悜锛氬厤鐤仐浼犲銆
鎷ユ湁瓒呰繃 25 骞寸殑鍏嶇柅瀛︺佸垎瀛愮敓鐗╁鍜屽熀鍥犲墧闄ょ被妯″紡鍔ㄧ墿鍒跺鍜岀爺绌剁粡楠岋紝鑷村姏浜庡埄鐢ㄦā寮忓皬榧犳ā鍨嬭繘琛岃偪鐦ゅ拰鑷韩鍏嶇柅鎬х柧鐥呰嚧鐥呮満鐞嗙殑鍩虹鐮旂┒銆鏇惧埄鐢ㄤ笉鍒颁袱骞寸殑鏃堕棿鐮斿彂鍑轰笘鐣屼笂鏈鏃╃殑IL17-EGFP銆IL-17-Gluc 鑽у厜铔嬬櫧鍜鑽у厜绱犻叾鎶ュ憡鍩哄洜宓屽叆妯″紡灏忛紶锛屾巿鏉冪粰鍏ㄧ悆鍑犲崄瀹跺ぇ瀛︺佺爺绌舵墍鍜屽尰鑽紒涓氾紝鍖呮嫭 NIH 鍜屽搱浣涘ぇ瀛︺傚悗甯﹂鍥㈤槦鎴愬姛寮鍙戯細1锛夋嫢鏈夌煡璇嗕骇鏉冪殑楂樻晥鍩哄洜缁勭紪杈戯紙EGE锛夌郴缁燂紱2锛夎嚜涓荤爺鍙戜紶浠ｇǔ瀹氱殑 C57BL/6 灏忛紶鑳氳儙骞茬粏鑳炪傚洟闃熷埄鐢浠ヤ笂鏍稿績鎶鏈埗澶囩殑楂樼妯″紡鍔ㄧ墿锛屽凡缁忓拰姝ｅ湪寮鍙戜富瑕佷骇鍝佸寘鎷熀鍥犵紪杈戝ぇ/ 灏忛紶杩 2000 浣欑锛屽熀鍥犵紪杈戠粏鑳炵郴 200 浣欑锛岄潪浜哄ぇ鍔ㄧ墿鍜岀伒闀跨被 4 绉嶃佹枒椹楸 5 绉嶃備互鍩哄洜缂栬緫妯″紡鍔ㄧ墿涓哄熀纭灏嗘湇鍔＄绫绘嫇灞曞埌鑽唬鑽姩銆佹瘨鐞嗙梾鐞嗙瓑棰鍩熴傚湪鎶椾綋鏂拌嵂鐮斿彂鏂归潰锛屽埄鐢ㄥ叕鍙稿熀鍥犵紪杈戝钩鍙般佸姩鐗╃箒娈栦笌渚涘簲骞冲彴鍜岃嵂鐞鑽晥鏈嶅姟锛屽叏娴佺▼浼樺寲鍜屾彁鍗囦簡浼犵粺鎶椾綋鍙戠幇娴佺▼锛屽疄鐜伴珮鏁堝揩鎹枫侀珮鎴愬姛鐜囩殑鎶椾綋鍙戠幇鍜岀瓫閫夈
浠ｈ〃鎬ц鏂锛
[1] Bioluminescent imaging of vaccinia virus infection in immunocompetent and immunodeficient
rats as a model for human smallpox. Journal of Infection & Chemotherapy, 2015, 5(6):
278-285.
[2] Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system. Scientific Reports, 2016, 6:24360.
[3] Visualization of mucosal homeostasis via single- and multiphoton intravital fluorescence
microscopy. Journal of Leukocyte Biology, 2012, 92(3): 413.
[4] CXCR4 acts as a costimulator during thymic β-selection. 2009, 11(2): 162-170.
[5] TGF-β-induced Foxp3 inhibits TH17 cell differentiation by antagonizing RORγt function.
Nature, 2008, 453(7192): 236.
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